Technology

Revolutionizing Microbiome Quantification with qMA™ Technology

At Branchpoint Biosciences, we’ve developed qMA™ - our groundbreaking quantitative Microbial Assays - to transform microbiome profiling through advanced qPCR technology. Our products offer rapid, accurate, and cost-effective quantification of target microorganisms, empowering researchers and clinicians to gain deeper insights and accelerate discoveries.

Key Advantages of qMA™ Technology

1. Precision Targeting with Phylogenetic Resolution

  • Strain- and Species-Level Detection: Our proprietary informatics pipeline designs primers and probes tailored to your desired level of phylogenetic resolution—whether at the strain, species, or genus level.
  • Customized Solutions: We leverage curated genome databases and a flexible design process to precisely target bacterial and archaeal taxa relevant to your research.

2. Superior Sensitivity

  • Detect Low-Abundance Microbes: Our qPCR-based assays reliably detect target organisms with as few as 10 copies per reaction, surpassing the sensitivity limitations of standard NGS methods.
  • High Throughput: Achieve efficient quantification across multiple samples, enhancing the scalability of your studies.

Figure Legend: Standard curves for three multiplexed targets (circles): Peptostreptococcus anaerobius, Bacteroides fragilis, and Fusobacterium nucleatum. Squares denote spike-ins within a natural, background stool community.

3. Easy Integration and Rapid Results

  • Compatibility: Our assays are designed for seamless integration with a variety of widely available qPCR platforms in academic, industry, and clinical laboratories.
  • User-Friendly: Eliminate the burden of complex bioinformatics analysis with established, pre-defined assays.
  • Fast Turnaround: Obtain actionable insights within hours, accelerating your research timelines compared to longer NGS pipelines.

4. Multiplexing Capability

  • Simultaneous Quantification: Utilize differentially labeled, sequence-specific fluorogenic probes to quantify multiple targets within a single reaction.
  • Comprehensive Analysis: Directly compare within-well abundances of target organisms, streamlining your workflow and reducing assay time.

5. Absolute Quantification

  • Exact Microbial Counts: Determine the actual number of target sequences present, providing absolute abundance measurements without the need for additional sequencing.
  • Enhanced Data Accuracy: Gain precise insights into microbial community composition, improving the reliability of your results.

6. Cost-Effective and Scalable

  • Significant Cost Savings: qMA assays are significantly less expensive than NGS, allowing you to reduce overall budgets or allocate resources to larger study cohorts.
  • Facilitate Larger Studies: Conduct trials at appropriate scales to validate bacterial biomarkers without compromising quality or accuracy.

How qMA™ Technology Can Advance Your Research

  • Custom Multiplexed Kits: Targeted profiling tailored to meet your specific research needs.
  • Body Site-Specific Panels: Rapidly identify microbiome dysbiosis or monitor changes due to interventions in oral, vaginal, and urogenital microbiomes.
  • Diagnostic Panels: Scale up cohorts and validate bacterial biomarkers with precision and confidence.
  • Strain-Specific Monitoring: Develop custom assays for monitoring proprietary Live Biotherapeutic Products with high specificity.

Our Commitment to Quality and Innovation

At Branchpoint Biosciences, we’re dedicated to providing high-quality, reliable assays that advance microbiome research and clinical applications. Our rigorous validation process ensures that our primers and probes meet the highest standards:

  • In Silico Validation: Utilizing our extensive proprietary database to assess specificity and performance.
  • In Vitro Assessment: Testing against a catalog of defined reference genetic material and within complex community samples to confirm efficacy and accuracy.

By choosing qMA™ technology, you’re partnering with a team committed to pushing the boundaries of microbiome analysis and supporting your journey toward groundbreaking discoveries.